pdcas9dnmt3a egfp (Addgene inc)
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pdcas9dnmt3a egfp
Pdcas9dnmt3a Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pdcas9+dnmt3a+egfp/pdCas9-DNMT3A-EGFP+(Plasmid+%2371666)/pm41620608-256-17-18
Average 93 stars, based on 33 article reviews
Pdcas9dnmt3a Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pdcas9+dnmt3a+egfp/pdCas9-DNMT3A-EGFP+(Plasmid+%2371666)/pm41620608-256-17-18
Average 93 stars, based on 33 article reviews
pdcas9dnmt3a egfp - by Bioz Stars,
2026-09
93/100 stars
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Plasmid Preparation:Article Title: Systematic comparison of dCas9-based DNA methylation epimodifiers over time indicates efficient on-target and widespread off-target effects Article Snippet: The same procedure was applied to the control tool, consisting of dCas9 fused with the inactive DNMT3A (M3-dCAS9-DNMT3A, Addgene #220237). .. M3-dCAS9-DNMT3A-DNMT3A (dCas9-3A-3A, Addgene #218776) plasmid made by the amplification of the human DNMT3A catalytic domain from the Article Title: The dCas9-based genome editing in Plasmodium yoelii Article Snippet: .. The guide RNA (gRNA) was designed upstream of the protospacer-adjacent motif (PAM), using the online CRISPR guide RNA/DNA design tool for eukaryotic pathogens (EuPaGDT; http://grna.ctegd.uga.edu ), and a pair of complementary oligonucleotides were synthesized for each target site (Sangon Biotech, China). dCas9 nuclease coding fragment was amplified from Article Title: VIM-AS1 , which is regulated by CpG methylation, cooperates with IGF2BP1 to inhibit tumor aggressiveness via EPHA3 degradation in hepatocellular carcinoma Article Snippet: .. Article Title: The dCas9-based genome editing in Plasmodium yoelii . Article Snippet: .. The guide RNA (gRNA) was designed upstream of the protospacer-adjacent motif (PAM), using the online CRISPR guide RNA/DNA design tool for eukaryotic pathogens (EuPaGDT; http://grna.ctegd.uga.edu), and a pair of complementary oligonucleotides were synthesized for each target site (Sangon Biotech, China). dCas9 nuclease coding fragment was amplified from Article Title: p21 Promoter Methylation Is Vital for the Anticancer Activity of Withaferin A Article Snippet: In experiments designed for targeted demethylation of promoters, we utilized pINDUCER dCas9-TET1CD, which was a gift from Danwei Huangfu (Addgene plasmid #101921; https://www.addgene.org/101921/ (accessed on 8 February 2024); RRID:Addgene_101921; RRID:Addgene_129025) [61]. .. In experiments designed for targeted methylation of promoters, we employed Article Title: p21 Promoter Methylation Is Vital for the Anticancer Activity of Withaferin A Article Snippet: In experiments designed for targeted demethylation of promoters, we utilized pINDUCER dCas9-TET1CD, which was a gift from Danwei Huangfu (Addgene plasmid #101921; https://www.addgene.org/101921/ (accessed on 8 February 2024); RRID:Addgene_101921; RRID:Addgene_129025) [ ]. .. In experiments designed for targeted methylation of promoters, we employed Amplification:Article Title: Systematic comparison of dCas9-based DNA methylation epimodifiers over time indicates efficient on-target and widespread off-target effects Article Snippet: The same procedure was applied to the control tool, consisting of dCas9 fused with the inactive DNMT3A (M3-dCAS9-DNMT3A, Addgene #220237). .. M3-dCAS9-DNMT3A-DNMT3A (dCas9-3A-3A, Addgene #218776) plasmid made by the amplification of the human DNMT3A catalytic domain from the Article Title: The dCas9-based genome editing in Plasmodium yoelii Article Snippet: .. The guide RNA (gRNA) was designed upstream of the protospacer-adjacent motif (PAM), using the online CRISPR guide RNA/DNA design tool for eukaryotic pathogens (EuPaGDT; http://grna.ctegd.uga.edu ), and a pair of complementary oligonucleotides were synthesized for each target site (Sangon Biotech, China). dCas9 nuclease coding fragment was amplified from Article Title: The dCas9-based genome editing in Plasmodium yoelii . Article Snippet: .. The guide RNA (gRNA) was designed upstream of the protospacer-adjacent motif (PAM), using the online CRISPR guide RNA/DNA design tool for eukaryotic pathogens (EuPaGDT; http://grna.ctegd.uga.edu), and a pair of complementary oligonucleotides were synthesized for each target site (Sangon Biotech, China). dCas9 nuclease coding fragment was amplified from Methylation:Article Title: Genome-wide DNA hypermethylation opposes healing in patients with chronic wounds by impairing epithelial-mesenchymal transition Article Snippet: .. Crisper/Cas9-mediated targeted DNA methylation/demethylation. Article Title: p21 Promoter Methylation Is Vital for the Anticancer Activity of Withaferin A Article Snippet: In experiments designed for targeted demethylation of promoters, we utilized pINDUCER dCas9-TET1CD, which was a gift from Danwei Huangfu (Addgene plasmid #101921; https://www.addgene.org/101921/ (accessed on 8 February 2024); RRID:Addgene_101921; RRID:Addgene_129025) [61]. .. In experiments designed for targeted methylation of promoters, we employed Article Title: p21 Promoter Methylation Is Vital for the Anticancer Activity of Withaferin A Article Snippet: In experiments designed for targeted demethylation of promoters, we utilized pINDUCER dCas9-TET1CD, which was a gift from Danwei Huangfu (Addgene plasmid #101921; https://www.addgene.org/101921/ (accessed on 8 February 2024); RRID:Addgene_101921; RRID:Addgene_129025) [ ]. .. In experiments designed for targeted methylation of promoters, we employed CRISPR:Article Title: The dCas9-based genome editing in Plasmodium yoelii Article Snippet: .. The guide RNA (gRNA) was designed upstream of the protospacer-adjacent motif (PAM), using the online CRISPR guide RNA/DNA design tool for eukaryotic pathogens (EuPaGDT; http://grna.ctegd.uga.edu ), and a pair of complementary oligonucleotides were synthesized for each target site (Sangon Biotech, China). dCas9 nuclease coding fragment was amplified from Article Title: The dCas9-based genome editing in Plasmodium yoelii . Article Snippet: .. The guide RNA (gRNA) was designed upstream of the protospacer-adjacent motif (PAM), using the online CRISPR guide RNA/DNA design tool for eukaryotic pathogens (EuPaGDT; http://grna.ctegd.uga.edu), and a pair of complementary oligonucleotides were synthesized for each target site (Sangon Biotech, China). dCas9 nuclease coding fragment was amplified from Synthesized:Article Title: The dCas9-based genome editing in Plasmodium yoelii Article Snippet: .. The guide RNA (gRNA) was designed upstream of the protospacer-adjacent motif (PAM), using the online CRISPR guide RNA/DNA design tool for eukaryotic pathogens (EuPaGDT; http://grna.ctegd.uga.edu ), and a pair of complementary oligonucleotides were synthesized for each target site (Sangon Biotech, China). dCas9 nuclease coding fragment was amplified from Article Title: The dCas9-based genome editing in Plasmodium yoelii . Article Snippet: .. The guide RNA (gRNA) was designed upstream of the protospacer-adjacent motif (PAM), using the online CRISPR guide RNA/DNA design tool for eukaryotic pathogens (EuPaGDT; http://grna.ctegd.uga.edu), and a pair of complementary oligonucleotides were synthesized for each target site (Sangon Biotech, China). dCas9 nuclease coding fragment was amplified from Construct:Article Title: The dCas9-based genome editing in Plasmodium yoelii Article Snippet: .. The guide RNA (gRNA) was designed upstream of the protospacer-adjacent motif (PAM), using the online CRISPR guide RNA/DNA design tool for eukaryotic pathogens (EuPaGDT; http://grna.ctegd.uga.edu ), and a pair of complementary oligonucleotides were synthesized for each target site (Sangon Biotech, China). dCas9 nuclease coding fragment was amplified from Article Title: The dCas9-based genome editing in Plasmodium yoelii . Article Snippet: .. The guide RNA (gRNA) was designed upstream of the protospacer-adjacent motif (PAM), using the online CRISPR guide RNA/DNA design tool for eukaryotic pathogens (EuPaGDT; http://grna.ctegd.uga.edu), and a pair of complementary oligonucleotides were synthesized for each target site (Sangon Biotech, China). dCas9 nuclease coding fragment was amplified from |
